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    Protocol

    Progenitor Biological Bandages: An Authentic Swiss Tool for Safe Therapeutic Management of Burns, Ulcers, and Donor Site Grafts

    Clinical experience gathered over two decades around therapeutic use of primary human dermal progenitor fibroblasts in burn patient populations has been at the forefront of regenerative medicine in Switzerland...

    Alexis Laurent, Corinne Scaletta, Murielle Michetti, Nathalie Hirt-Burri (2020)

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    The Characteristics of Human iPS Cells and siRNA Transfection Under Hypoxia

    The characteristics of pluripotent cells have great potential for basic and clinical research and application. We describe the effect of normoxia or hypoxia regarding the proliferation and pluripotency of huma...

    Kouji Sugimoto, Yoshihiko Hayashi (2020)

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    mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions

    2020欧洲杯体育官方网站 Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for...

    Jakob Jeriha, Nikola Kolundzic, Preeti Khurana, Andrea Perez-Dominguez, Dusko Ilic (2020)

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    2020欧洲杯体育官方网站 Protocol

    Generation of Monoclonal iPSC Lines with Stable Cas9 Expression and High Cas9 Activity

    CRISPR/Cas9-mediated gene editing has been rapidly and widely applied in many organisms for delicate genetic manipulation, including human-induced pluripotent stem cells (iPSCs). Gene editing in human iPSCs is...

    Jin Qi Liao, Guangqian Zhou, Yan Zhou (2020)

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    2020欧洲杯体育官方网站 Protocol

    Chemical and Voltage Gating of Gap Junction Channels Expressed in Xenopus Oocytes

    2020欧洲杯体育官方网站 In most tissues, cells in contact with each other directly intercommunicate via cell-to-cell channels aggregated at gap junctions. Direct cell-to-cell communication provides a fundamental mechanism for coordin...

    Camillo Peracchia (2020)

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    Ex Vivo Modeling of Hematopoietic Stem Cell Homing to the Fetal Liver

    2020欧洲杯体育官方网站 Hematopoietic stem cells (HSCs) are used in the clinic to provide life-saving therapies to patients with a variety of hematological malignancies and disorders. Yet, serious deficiencies in our understanding of...

    Amina Mohammadalipour, Miguel F. Diaz, Sumedha Pareek, Pamela L. Wenzel (2020)

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    Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Endometrial Stem Cells for Multiple Sclerosis

    2020欧洲杯体育官方网站 Multiple sclerosis (MS) is the most common cause of neurological diseases. Although, there are some effective medications with regulatory approval for treating MS, they are only partially effective and cannot ...

    Somayeh Ebrahimi-Barough, Jafar Ai, Moloud Payab, Sepideh Alavi-Moghadam (2020)

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    GMP-Compliant Human Schwann Cell Manufacturing for Clinical Application

    2020欧洲杯体育官方网站 Schwann cells as glial cells in the peripheral nervous system can participate in neurons protection and forming myelin. Additionally, they are important for nerve pulse conduction supporting along axons. On th...

    Babak Arjmand, Parisa Goodarzi, Sepideh Alavi-Moghadam, Moloud Payab (2020)

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    GMP-Compliant Production of Human Placenta-Derived Mesenchymal Stem Cells

    Mesenchymal stem cells are one of the most attractive sources for stem cell research and therapy. Their safety and efficacy have been demonstrated in many clinical trials. Because of their low immunogenicity a...

    Hamid Reza Aghayan, Moloud Payab, Fereshteh Mohamadi-Jahani, Seyed Sajjad Aghayan (2020)

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    GMP-Compliant Adenoviral Vectors for Gene Therapy

    Recently, gene therapy as one of the most promising treatments can apply genes for incurable diseases treatment. In this context, vectors as gene delivery systems play a pivotal role in gene therapy procedure....

    Babak Arjmand, Sepideh Alavi-Moghadam, Moloud Payab, Parisa Goodarzi (2020)

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    Interactions of Hematopoietic Stem Cells with Bone Marrow Niche

    Hematopoietic stem cells (HSCs) are ultimately responsible for the lifelong renewal of all blood cell lineages. In the bone marrow (BM), HSCs reside in specialized microenvironments referred to as the “niche.”...

    Xinghui Zhao, Cuiping Zhang, Xiaojing Cui, Ying Liang (2020)

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    Calcium Fluorescence Recordings from Neuroepithelial Stem Cells

    Neuroepithelial cells act as neural stem cells by renewing themselves during embryonic development. These cells are tightly interconnected and make contact with the basement membrane of the neuroepithelium. Un...

    Masayuki Yamashita (2020)

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    Scalable Manufacturing of Human Hematopoietic Stem/Progenitor Cells Exploiting a Co-culture Platform with Mesenchymal Stromal Cells

    In the context of hematopoietic cell transplantation, hematopoietic stem/progenitor cells (HSPC) from the umbilical cord blood (UCB) present several advantages compared to adult sources including higher prolif...

    Ana Fernandes-Platzgummer, Pedro Z. Andrade, Joaquim M. S. Cabral (2020)

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    2020欧洲杯体育官方网站 Protocol

    GMP Tiered Cell Banking of Non-enzymatically Isolated Dermal Progenitor Fibroblasts for Allogenic Regenerative Medicine

    Non-enzymatically isolated primary dermal progenitor fibroblasts derived from fetal organ donations are ideal cell types for allogenic musculoskeletal regenerative therapeutic applications. These cell types ar...

    Alexis Laurent, Corinne Scaletta, Murielle Michetti, Nathalie Hirt-Burri (2020)

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    Addressing Manufacturing Challenges for Commercialization of iPSC-Based Therapies

    2020欧洲杯体育官方网站 The development of reprogramming technology to generate human induced pluripotent stem cells (iPSCs) has tremendously influenced the field of regenerative medicine and clinical therapeutics where curative cell...

    Mehdi Dashtban, Krishna Morgan Panchalingam, Mehdi Shafa, Behnam Ahmadian Baghbaderani (2020)

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    Swiss Fetal Transplantation Program and Non-enzymatically Isolated Primary Progenitor Cell Types for Regenerative Medicine

    Primary progenitor cell types adequately isolated from fetal tissue samples present considerable therapeutic potential for a wide range of applications within allogeneic musculoskeletal regenerative medicine. ...

    Alexis Laurent, Corinne Scaletta, Nathalie Hirt-Burri, Wassim Raffoul (2020)

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    Equilibrium Sampling of Hydrophobic Organic Contaminants in Sediments

    Passive samplers are useful novel tools for assessing contaminant exposure and evaluating its potential for adverse environmental effects. Passive equilibrium samplers enable the quantification of freely disso...

    Gesine Witt, Julia Bachtin, Sabine Schäfer (2020)

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    A Co-culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival

    Methods for the mechanistic investigations on renal fibrosis have long been concentrated on individual type of cells, such as fibroblasts and epithelial cells. However, in recent years, growing numbers of stud...

    Ling Lin, Samantha White, Kebin Hu (2020)

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    Whole-Sediment Toxicity Bioassay to Determine Bioavailability and Effects of Aquatic Contaminants Using Zebrafish Embryos

    Sediments are a major issue for many scientists and authorities due to their close linkage to the aquatic food web and their potential to accumulate pollutants. In the present chapter, the method of exposure o...

    Sabrina Schiwy, Mirna Velki, Henner Hollert (2020)

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    Passive Sampling of Waterborne Contaminants

    Passive sampling is a method that can conveniently be used for monitoring of organic contaminants in the aquatic environment comprising its various compartments. The time-integrating properties of passive samp...

    Branislav Vrana, Foppe Smedes, Klára Hilscherová (2020)

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